Biological Additive Manufacturing Capabilities for Enabling Rapid Response to Emerging Pathogens

Matthew Coleman | 21-ERD-039

Executive Summary

We will develop a first-of-its-kind engineered platform to perform protein production, binding, and affinity measurements in a fraction of the time required by current methods. The technology will provide a truly rapid response pipeline for producing and investigating biothreat-related proteins such as Ebola viruses.

Publications, Presentations, and Patents

Chao Liu, Steven Hoang-Phou, Congwang Ye, Matthew Laurence, Bonnee Rubinfeld, Byron Dillon Vannest, Thomas Desautels, William Smith, Erika Fong, Nicholas Watkins, Sean Gilmore, Maxim Shusteff, Ted Laurence, Matthew Coleman. "Fluorescence Correlation Spectroscopy of Proteins Expressed inside Microcapsules Developing a High Throughput Cell-free System for Generating and Characterizing Protein-based Countermeasures." Biophysical Society, San Francisco, CA. February 2022.

Congwang Ye, Steven Hoang-Phou, Nikki Hammond, Chao Liu, Erika Fong, Nicholas Watkins, Matthew Laurence, William Smith, Sean Gilmore, Ted Laurence, Daniel Faissol, Matthew Coleman. "One-step Microencapsulation of Cell-free Lysate for Improved Protein Synthesis and Characterization." Protein Society. San Francisco, CA. July 2022.

Laurence, M.J., Carpenter, T.S., Laurence, T.A., Coleman, M.A., Shelby, M. and Liu, C. 2022. "Biophysical Characterization of Membrane Proteins Embedded in Nanodiscs using Fluorescence Correlation Spectroscopy." Membranes. 12(4), 392;