Revealing Molecular and Cellular Mechanisms of Microbial Pathogenesis Through Multiscale Dynamic Imaging

Executive Summary

This project will establish the Lattice Light Sheet Microscope, a dynamic imaging technology, as a critical tool to study multiple length and timescales of protein structures and functions in infectious disease organisms that pose global public health threats. Understanding the time sequence of subcellular molecular events using this technology will provide a powerful aid in developing pathogen countermeasures.

Publications, Presentations, and Patents

Yi, Xiyu, Haichao Miao, Jacky Kai-Yin Lo, Wes Overton, Tek Hyung Lee, Maher M. Elsheikh, Dante Paul Ricci, Dan Mcfarland Park, Peet-Timo Bremer, Brent W. Segelke, Ted A. Laurence. "Commissioning a Home-Built Lattice Light Sheet Microscope for the Study of Legionella Infection." 66th Biophysical Society Annual Meeting. San Francisco CA. February 19-23, 2022.

Segelke, Brent W. 2022. "Functional Annotation from Structural Homology." In Microbial Systems Biology, pp. 215-257. Humana, New York, NY.

Yi, Xiyu, Haichao Miao, Jacky Kai-yin Lo, Maher M. Elsheikh, Tek-Hyung Lee, Chenfanfu Jiang, Yuliang Zhang, Brent W. Segelke, K. Wesley Overton, Peer-Timo Bremer, and Ted A. Laurence. 2022. "Tailored Approach to Study Legionella Infection Using a Lattice Light Sheet Microscope." (LLSM). Biomedical Optics Express 13, no. 8 2022: 4134-4159.