Rapid Fabrication of Engineered Gradient Tissue Scaffolds Using Cell-Embedded Microbeads

Executive Summary

We will develop a novel capability to produce high-throughput, tunable, highly controlled, cell-laden, microfabricated hydrogel beads (microbeads) leveraging a unique advanced manufacturing system known as the In-air Drop Encapsulation Apparatus and apply it to living, human systems for the first time. We will engineer the first-ever gradient tissue using cell-embedded microbeads and develop the framework to fabricate customizable living microscale constructs that can be used in applications such as biosensing and bioremediation, as well as biofuel and biotherapeutic production.

Publications, Presentations, and Patents

Wasson, E.M. et al. "Life by the Drop: Engineering Human Gradient Tissues Using Cell-Embedded Microbeads." LLNL Slam presentation, September 2022. LLNL-PRES-839697.

Le-Doux, C. "Microbead Image Analysis for Rapid Counting and Measurement." LLNL Slam presentation, August 2022. LLNL-PRES-838019.